Food Science and Technology Department


Date of this Version



Published in Bioresource Technology 304 (2020), 122953 doi:10.1016/j.biortech.2020.122953


Copyright © 2020 Elsevier Ltd. Used by permission.


Wastewater with 0.2, 0.4, 0.8, 1.0 mg/L free chlorine was biologically treated using co-immobilized microalgae/ bacteria. In contrast, non-pretreated wastewater was treated with beads (control) and blank beads (blank) under the same operating condition. Results showed that NaClO pretreatment removed 8–33% total nitrogen (TN), 31–45% true color and 0.7–2.5 log CFU/mL aerobic-bacteria. At the end of treatment, maximum algal biomass (2,027 dry weight mg/L) was achieved with 0.2 mg/L free chlorine. Bacterial growth in wastewater was decreased by NaClO pretreatment before reaching 7.2–7.7 log CFU/mL on the fifth day. Beads with microorganisms (control) removed 15% more chemical-oxygen-demand (COD), 16% more TN, and 13% more total phosphate (PO43−) than blank. Pretreatment with 0.2 mg/L free chlorine increased TN removal from 75% to 80% while pollutants removal was substantially decreased with 0.4–1.0 mg/L free chlorine. Considering algal biomass growth and pollutants removal, 0.2 mg/L free chlorine pretreatment was recommended for microalgae/ bacteria co-immobilized system.