Polyphenolic fractions isolated from red raspberry whole fruit, pulp, and seed differentially alter the gut microbiota of mice with diet-induced obesity

Authors

Yibo Xian, University of Nebraska-Lincoln
Rong Fan, University of Nebraska-Lincoln
Jing Shao, University of Nebraska-Lincoln
Ashley Mulcahy Toney, University of Nebraska-Lincoln
Soonkyu Chung, University of Nebraska-Lincoln; University of Massachusetts AmherstFollow
Amanda E. Ramer-Tait, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

2020

Citation

https://doi.org/10.1016/j.jff.2020.104288

Comments

Available online 26 November 2020 1756-4646/© 2020 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Journal of Functional Foods 76 (2021) 104288

Abstract

Polyphenol extracts from red raspberry (RR) whole fruit or pulp, but not seed, attenuate high-fat (HF) dietinduced obesity in mice. Because host metabolism is linked to the microbiota, we investigated the effects of polyphenols from RR fruit, pulp, and seed on the microbiome. RR polyphenols significantly decreased the abundance of specific taxa that were increased during HF feeding relative to a low-fat diet, including Ruminococcus and an unclassified genus from Clostridiales. Compared to a HF diet, pulp and seed polyphenols increased Roseburia abundance and decreased levels of an unclassified genus from Mogibacteriaceae. RR seed polyphenols uniquely increased Bifidobacterium compared to a HF diet. The most notable taxon driving differential abundance among all diets was an unclassified genus from Coriobacteriaceae. Importantly, host metabolic markers improved by pulp polyphenols were strongly correlated with select microbiome features, indicating that specific gut bacteria may be involved in RR polyphenol catabolism and/or mediating health benefits.