Food Science and Technology Department


Date of this Version

May 1986


Published in APPLIED AND ENVIRONMENTAL MICROBIOLOGY, May 1986, p. 1121-1123. Copyright © 1986, American Society for Microbiology. Used by permission.


Clostridium acetobutylicum cells, when energized with fructose, transported and phosphorylated the glucose analog 2-deoxyglucose by a phosphoenolpyruvate-dependent phosphotransferase (PT) system. Butanol up to 2% did not inhibit PT activity, although its chaotropic effect on the cell membrane caused cellular phosphoenolpyruvate and the 2-deoxyglucose-6-phosphate to leak out. Cells harvested from the solventogenic phase of batch growth had a significantly lower PT activity than did cells from the acidogenic phase.

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