Food Science and Technology Department


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Published in APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 1983, p. 233-236 Vol. 46, No. 1. 0099-2240/83/070233-04$02.00/0 Copyright © 1983, American Society for Microbiology. Used by permission.


A filter paper replication system particularly useful for isolation of radiation-sensitive mutants of pigmented bacteria was devised. The fidelity of replication was high. Adhesion between a paper disk and a properly dried master plate provided adequate contact pressure. The replicas arising from this technique constitute a convenient apparatus for general application in isolation of clones sensitive to a discriminating treatment.

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