Honors Program


Date of this Version

Spring 3-18-2020

Document Type



Thomas, R. Remediation Strategies to Reduce Heavy Metal Uptake in Lettuce Grown in Contaminated Urban Soil. 2020. Undergraduate Honors Thesis. University of Nebraska-Lincoln.


Copyright Ryley Thomas 2020.


Urban soils are increasingly used to produce food for local consumption, which requires at the same time management strategies that prevent the plant uptake of potentially present contaminants. This study was conducted to test different soil amendments for their ability to retain lead (Pb) and arsenic (As) within the soil matrix. The analyzed soil was taken from a potential community garden lot near a railroad in Lincoln, NE where elevated concentrations in Pb and As had been detected. Ponderosa pine biochar and spent coffee grounds were used as soil additives because of their documented chemical reactivity towards soil cations or anions. In a greenhouse experiment, romaine lettuce (Lactuce sativa) was grown in the soil collected from the community garden location with or without addition of biochar or coffee grounds. Lettuce was chosen due to its short growing season and high relevance for urban food production. The biochar amendment resulted in the lowest concentrations of both Pb and As in the leaves and stems parts of the plant compared to the garden control and the coffee grounds amendment. In contrast, the coffee grounds had the highest concentrations of Pb and As throughout all three tested parts of the plant (leaf, stem, and roots) across treatments. These findings suggest that biochar application to urban soil presents a potential successful strategy to prevent the plat uptake of contaminants such as As and Pb. In a next step, this strategy needs to be tested for its usefulness towards a large variety of plants grown for food production in the urban environment.