The Effects of Temperature and Mito-Nuclear Interaction on Male Fertility in Drosophila.

Authors

Violetta Bakunina, University of Nebraska-LincolnFollow

Date of this Version

Spring 3-27-2023

Document Type

Thesis

Citation

Bakunina,Violetta (2023).The Effects of Temperature and Mito-Nuclear Interaction on Male Fertility in Drosophila. Undergraduate Honors Thesis. University of Nebraska-Lincoln.

Comments

Published: University of Nebraska-Lincoln, Nebraska

Copyright Violetta Bakunina 2023.

Abstract

The (simw⁵⁰¹);Ore genotype is a strain of Drosophila melanogaster that has impaired mitochondrial function as a result of a disrupted interaction between a mitochondrially-encoded tRNA^Tyr and the nuclear-encoded synthetase that aminoacylates mt-tRNATyr. We hypothesized that disruption of mitochondrial protein synthesis and oxidative phosphorylation (OXPHOS) in this genotype decreases mitochondrial translation and results in the sterility of (simw⁵⁰¹);OreR males at 28^oC. The results of Mitotracker staining showcased the reduced Mean Fluorescence Intensity (MFI), which confirms that sterility has an energetic basis, yet also that mature sperm function is compromised. Cytological dissection of male gonads revealed consistent morphological differences between (simw⁵⁰¹);OreR and control genotypes, such as reduced seminal vesicle area, expanded sperm individualization testis site and failure of sperm to undergo proper individualization. Energetic deficiencies in mature sperm, along with physiological abnormalities during spermatogenesis, represent that (simw⁵⁰¹);OreR does not meet the demands of the energy-costly process of spermatogenesis and thus fails to produce viable gamete. These factors together may be the underlying reason for male infertility of (simw⁵⁰¹);OreR genotype. The incompatible (simw⁵⁰¹);OreR genotype serves as a model for deciphering how mitochondrial dysfunction contributes to human disease and considering the benefits and costs of mitochondrial replacement therapy and novel treatments.