Comparative gene mapping: cytogenetic localization of PROC, EN1, ALPI, TNP1, and IL1B in cattle and sheep reveals a conserved rearrangement relative to the human genome

Authors

N. L. Lòpez-Corrales, Roslin Institute (Edinburgh), Roslin, Midlothian, Edinburgh
T. S. Sonstegard, USDA/ARS Beltsville Agricultural Research Center, Beltsville, MD
T. P. L. Smith, U.S. Meat Animal Research CenterFollow

Date of this Version

1998

Document Type

Article

Citation

Cytogenet Cell Genet 83:35–38 (1998)

Abstract

The cytogenetic locations of the genes for protein C (PROC), transition protein 1 (TNP1), intestinal alkaline phosphatase (ALPI), engrailed (EN1), and human protointerleukin ß (IL1B) have been compared between cattle (Bos taurus, BTA) and sheep (Ovis aries, OAR). Bovine YAC and cosmid clones were used as FISH probes to determine the order (centromere to telomere) of four of these genes on OAR 2q, as well as the location of IL1B on OAR 3p. In cattle, IL1B and EN1 were assigned to BTA 11 and BTA 2, respectively. Alignment of the ovine, bovine, and human physical maps based on these data shows that segments of conserved synteny and chromosomal rearrangements detected between cattle and human are also found in sheep, where the order in cattle is conserved.