Cytokine and Haptoglobin Profiles From Shipping Through Sickness and Recovery in Metaphylaxis- or Un-Treated Cattle

Authors

Carol G. Chitko-McKown, USDA ARS Roman L. Hruska U.S. Meat Animal Research CenterFollow
Gary L. Bennett, USDA ARS Roman L. Hruska U.S. Meat Animal Research Center
Larry A. Kuehn, USDA ARS Roman L. Hruska U.S. Meat Animal Research Center
Keith D. DeDonder, Kansas State University
Michael D. Apley, Kansas State University
Gregory P. Harhay, USDA ARS Roman L. Hruska U.S. Meat Animal Research CenterFollow
Michael L. Clawson, USDA ARS Roman L. Hruska U.S. Meat Animal Research Center
Aspen M. Workman, USDA ARS Roman L. Hruska U.S. Meat Animal Research CenterFollow
Bradley J. White, Kansas State University
Robert L. Larson, Kansas State University
Sarah F. Capik, Kansas State University
Brian V. Lubbers, Kansas State University

Date of this Version

3-19-2021

Document Type

Article

Citation

Chitko-McKown CG, Bennett GL, Kuehn LA, DeDonder KD, Apley MD, Harhay GP, Clawson ML, Workman AM, White BJ, Larson RL, Capik SF and Lubbers BV (2021) Cytokine and Haptoglobin Profiles From Shipping Through Sickness and Recovery in Metaphylaxis- or Un-Treated Cattle. Front. Vet. Sci. 8:611927. doi: 10.3389/fvets.2021.611927

Comments

This is an open-access article distributed under the terms of the Creative Commons Attribution License

Abstract

Fifty-six head of cattle, 28 animals with bovine respiratory disease complex (BRDC), and 28 healthy animals that were matched by treatment, sale barn of origin, day, and interactions among these variables, were identified from a population of 180 animals (60 each purchased at three sale barns located in Missouri, Tennessee, and Kentucky) enrolled in a study comparing animals receiving metaphylaxis to saline-treated controls. Cattle were transported to a feedlot in KS and assigned to treatment group. Blood samples were collected at Day 0 (at sale barn), Day 1, Day 9, and Day 28 (at KS feedlot), and transported to the US Meat Animal Research Center in Clay Center, NE where plasma was harvested and stored at −80°C until assayed for the cytokines IFN-γ, IL-1β, IL-6, and TNF-α, and the acute stress protein haptoglobin (HPT). Our objectives were to determine if cytokine and haptoglobin profiles differed between control and metaphylaxis treatment groups over time, and if profiles differed between animals presenting with BRDC and those that remained healthy. There was no difference between the treated animals and their non-treated counterparts for any of the analytes measured. Sale barn of origin tended to affect TNF-α concentration. Differences for all analytes changed over days, and on specific days was associated with state of origin and treatment. The Treatment by Day by Case interaction was significant for HPT. The analyte most associated with BRDC was HPT on D9, possibly indicating that many of the cattle were not exposed to respiratory pathogens prior to entering the feedlot.