United States Department of Agriculture: Animal and Plant Health Inspection Service
United States Department of Agriculture Wildlife Services: Staff Publications
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Document Type
Article
Date of this Version
January 2005
Abstract
An analytical method to evaluate propionylpromazine hydrochloride (PPZHCl) in tranquilizer formulations was developed using high-performance liquid chromatography (HPLC). During analysis of aged quality-control samples, a previously unreported chromatographic response was observed at a shorter retention time than PPZHCl. Further investigation of formulations stored in trap tap devices at temperatures ranging from 5 to 40 ◦C during field trials at four different locations confirmed the degradation of the active ingredient. Further investigation using HPLC/tandem mass spectrometry revealed two to five degradates, with the major degradates being oxidation products of the active ingredient, PPZHCl. As PPZHCl formulations must be stable when stored at 5 to 40 ◦C for 6 to 12months, reformulation with the anti-oxidant ascorbic acid was utilized to achieve the required PPZHCl stability. Published in 2005 for SCI by John Wiley & Sons, Ltd.