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Insecta Mundi

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Document Type

Article

Date of this Version

2015

Citation

Insecta Mundi 0446: 1–5

Comments

Published in 2015 by Center for Systematic Entomology, Inc. P. O. Box 141874 Gainesville, FL 32614-1874 USA http://centerforsystematicentomology.org/. Copyright held by the author(s). This is an open access article distributed under the terms of the Creative Commons, Attribution Non-Commercial License

Abstract

Se presenta un protocolo para preparar material de Coccoidea (Insecta: Hemiptera) para realizar observaciones con microscopía electrónica de barrido. Esta técnica consiste en muestras conservadas en alcohol 96°, limpiadas con xilol y acetona y llevadas directamente a la etapa de hidratación y una posterior deshidratación, en alcohol, omitiendo la fi jación en formaldehído gluteraldehído y la posfijación en tetróxido de osmio. Se destaca la importancia de la limpieza del material y la correcta realización de punto crítico, en la etapa de desecación.

A new protocol to prepare material of Coccoidea (Insecta: Hemiptera) for observation with scanning electron microscopy is presented. This technique consists of cleaning samples (stored in 96% alcohol) with acetone and xylene, taking them directly to the hydration step, and subsequently dehydrating them in alcohol. Fixation in glutaraldehyde and post-fixation in osmium tetroxide are omitted. The cleaning of the samples and the correct critical point dryer are very important.

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