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The study presents the growth trend about authorship pattern and author collaboration in the Biotechnology for sixteen years with the sample of 18918 articles which have been collected from Scopus database during year 2001-2016. A predefined search string has been used for data download. The five scientometric tools collaboration coefficient (CC), authorship pattern (AP), activity index (AI), Relative Growth Rate (RGR) and Doubling time of Literature (Dt.) have been used for the data analysis. The average number of authors per article for India has been counted as 4.92. In the study, the collaboration coefficient for 16 years (2001-2016) is noticed as 0.63 for India. The study shows that multi- authorship articles are higher in average and they are dominant over single authorship pattern. The science growth rate is always useful for the study, so that relative growth rate and doubling time of literature tools have been used for the study to explore needs of manpower and finance for future and present research activity. The mean of relative growth rate for sixteen year shows the decreasing rate and for last four years its pattern of publication is stable. The corresponding doubling time for different years [Dt (P)] gradually fallowed the increasing pattern. It has been found that majority of the Biotechnology researchers are working through team research or collaborative research rather than individual research. The average activity index of India for sixteen years is counted as 91.78. The highest AI year for India is 2016 with 180.3 whereas the lowest counted in 2001 with 42.38. The international collaboration pattern shows for India, United States is the most favoured nation.