The study conducted to find the adaption of open access (OA) publishing among the research community of Tumkur University by sourcing the data from Web of Science (WoS) database. An overall 516 articles were published by the faculty of TU between 2011 and 2020 in 203 journals indexed in Web of Science database. 19.57% articles were available in pure OA routes and 63.17% funded articles published in gold route. A significant difference was found between citations of OA & Non-OA articles in last ten years. TU researchers have published articles with other researchers viz 22 articles with Americans, 18 with Canadians, 10 with Taiwanese, 9 with Germans, 5 articles with Chinese and 5 publications with Swedish authors. The top ranked journal “Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy” has published 9.88% followed by the “Journal of Science: Advanced Materials and Devices” published absolute number of articles in OA (15.84%). A significant contribution from the subjects like chemistry (186 articles) and material science (160 articles) was found. An investigation into the percentage of articles published through OA and non-OA routes was done to find out the level of citation impact both routes using “t” test. The results show OA articles mean value 17.32 and non-OA articles mean value of 18.03 at 1759 citations for 101 OA articles and 7483 citations for 415 non OA articles at a p-value of .004 levels between the two routes of publishing which is significant.