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The study evaluated the effects of job stress on job performance of librarians in selected public university libraries in Nigeria. It employed descriptive research design with the population of two hundred and sixty (260) librarians from all the public university libraries in South West, Nigeria. A questionnaire on the survey of job stress and effects on job performance of librarians was raised and validated. The reliability of the instrument was ascertained using Cronbach Alpha reliability to estimate the internal consistency of the instrument. The split half reliability was employed using 30 librarians from Nnamdi Azikwe Library, University of Nigeria, Nsukka, and it yielded 0.78. One hypothesis and two research questions were formulated and answered. The frequency counts and standard deviation were used to analyze the data. The findings revealed that there is high level of job stress experienced among librarians in the university libraries in South West, Nigeria. The study also established that there is a negative significant relationship between job stress and job performance, implying that increased job stress actually contributing to the drop in the job performance of librarians in university libraries in Nigeria. Based on these findings, library managers are encouraged to regularly organize conferences, workshops, seminars and other form of training on the effective management of stress for the purpose of increasing the level of job performance of librarians and librarians’ attendance to such training should be made compulsory.