North American Crane Working Group

 

Date of this Version

2022

Document Type

Article

Citation

PROCEEDINGS OF THE NORTH AMERICAN CRANE WORKSHOP 15:150-154: 2022

Comments

Used by permission.

Abstract

The management of ex situ and in situ populations of many wildlife species requires detailed knowledge of a species’ reproductive biology. For species such as cranes, where artificial insemination is a critical component of ex situ management strategies, understanding normal sperm function is especially important. Previous research has shown that captive cranes exhibit highly variable production and quality of semen samples produced by individual males and high levels of variation of cell concentration and motility across different species. Cyclic adenosine monophosphate (cyclic AMP) has been implicated in regulating sperm function, such as cell motility, and may affect an individual’s ability to successfully fertilize. Here we demonstrate the feasibility of an enzyme-linked immunosorbent assay (ELISA) for measuring cyclic AMP produced by crane sperm to facilitate future research into its role in sperm function and fertilization.

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