Wildlife Damage Management, Internet Center for
Date of this Version
5-25-2012
Citation
Invasive Species Advisory Committee, approved on May 25, 2012
Also available at https://www.doi.gov/sites/doi.gov/files/uploads/isac_pcr_white_paper.pdf
Abstract
This white paper provides:
a) Background information on the use, accuracy and reliability of PCR-based assays such as environmentally sampled DNA (eDNA) for early detection of aquatic invasive species (AIS) and;
b) Recommendations for establishing a system for validating assays and accrediting laboratories that report on the presence or absence of AIS.
This white paper was developed by the members of ISAC and discusses the need for developing validation requirements for Polymerase Chain Reaction (PCR) and other DNA-based molecular assays that are increasingly being used to detect AIS. It does not provide a simplified checklist for evaluation of their ability to detect AIS. Rather, it is intended to demonstrate the need for a required and regulated framework to validate these molecular assays. A regulated framework for validation would greatly increase confidence in the utility of DNA-based assays and better enable decision-makers and managers regarding AIS detection, prevention, monitoring and control.
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Aquaculture and Fisheries Commons, Biodiversity Commons, Bioinformatics Commons, Environmental Health and Protection Commons, Environmental Indicators and Impact Assessment Commons, Environmental Law Commons, Other Animal Sciences Commons, Research Methods in Life Sciences Commons, Sustainability Commons, Weed Science Commons
Comments
United States government work