National Park Service
ORCID IDs
http://orcid.org/0000-0003-3547-3062
Date of this Version
11-16-2018
Citation
Conservation Genetics Resources (2020) 12:109–123 https://doi.org/10.1007/s12686-018-1071-7
Abstract
Accurate and timely data regarding freshwater fish communities is important for informed decision-making by local, state, tribal, and federal land and resource managers; however, conducting traditional gear-based fish surveys can be an expensive and time-consuming process, particularly in remote areas, like those that characterize much of Alaska. To help address this challenge, we developed and tested five multi-species environmental DNA (eDNA) primer sets for the simultaneous detection of up to 37 target fish species in a single sample. Using these primer sets can reduce the cost and time needed to perform future studies of fish communities. Our results comparing multiple samples from multiple lakes and streams using multiple next-generation sequencing runs show the efficacy and reproducibility of these primers.
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Comments
U.S. Government Works are not subject to copyright.