Natural Resources, School of

School of Natural Resources: Dissertations, Theses, and Student Research
First Advisor
Leon Higley
Committee Members
Amanda Roe, Christian Elowsky
Date of this Version
5-2025
Document Type
Thesis
Citation
A thesis presented to the faculty of the Graduate College at the University of Nebraska in partial fulfillment requirement for the degree of Master of Science
Major: Natural Resource Sciences
Under the supervision of Professor Leon Higley
Lincoln, Nebraska, May 2025
Abstract
Forensic entomology scientists use blow flies' developing patterns to estimate the postmortem interval (PMI). However, larval aggregations produce metabolic heat, resulting in complex microclimates that may change growth timetables. This study investigates how larval density and associated temperature changes affect the development and survival of two forensically essential blow fly species, Lucilia sericata and Calliphora vicina.
This study examined how larval density and associated thermal profiles affect the development of both blow fly species. Using a split-plot design, we tested four larval densities at 25°C and 30°C, with temperature gradients measured via thermocouple at four mass positions three times daily. We recorded the number of pupae and measured the larvae’s weight to assess the extent of the density effect on the blow flies studied.
Key findings revealed density-dependent developmental patterns, with 1,000 larvae representing an optimal threshold where thermoregulatory benefits balance competition costs. Temperature gradients showed edge-to-center differentials up to 5.2°C, yet high-density masses exhibited prolonged development despite warmer microclimates. L. sericata demonstrated greater thermal tolerance than C. vicina, particularly at 30°C. Lethal temperature assays revealed species-specific survival thresholds, with preconditioning enhancing heat resistance in L. sericata but not C. vomitoria.
These results demonstrated that maggot mass temperature might not be reliable, as it may overestimate the developmental rate. We recommend a bigger container for maggot mass-related studies, starting with 1,000 larvae per container. The study provides a framework for density-adjusted ADD models and highlights climate change implications for blow fly communication dynamics in forensics contexts.
Advisor: Leon Higley
Included in
Earth Sciences Commons, Entomology Commons, Environmental Sciences Commons, Evolution Commons, Forensic Science and Technology Commons
Comments
Copyright 2025, Akomavo Fabrice Gbenonsi. Used by permission