Gas exchange measurements in the unsteady state

Authors

Aaron J. Saathoff, University of Nebraska-LincolnFollow
Jonathan Mark Welles, University of Nebraska - Lincoln

Document Type

Article

Date of this Version

2021

Citation

Plant Cell Environ. 2021;44:3509–3523.

DOI: 10.1111/pce.14178

Comments

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License

Abstract

Leaf level gas exchange is a widely used technique that provides real-time measurement of leaf physiological properties, including CO₂ assimilation (A), stomatal conductance to water vapour (g_sw) and intercellular CO₂ (C_i). Modern open-path gas exchange systems offer greater portability than the laboratory-built systems of the past and take advantage of high-precision infrared gas analyzers and optimized system design. However, the basic measurement paradigm has long required steadystate conditions for accurate measurement. For CO₂ response curves, this requirement has meant that each point on the curve needs 1–3 min and a full response curve generally requires 20–35 min to obtain a sufficient number of points to estimate parameters such as the maximum velocity of carboxylation (V_c,max) and the maximum rate of electron transport (J_max). For survey measurements, the steady-state requirement has meant that accurate measurement of assimilation has required about 1–2 min. However, steady-state conditions are not a strict prerequisite for accurate gas exchange measurements. Here, we present a new method, termed dynamic assimilation, that is based on first principles and allows for more rapid gas exchange measurements, helping to make the technique more useful for high throughput applications.