"Nondestructive Estimation of Leaf Chlorophyll Content in Grapes" by Mark Steele, Anatoly A. Gitelson et al.

Natural Resources, School of

 

Document Type

Article

Date of this Version

2008

Comments

Published by Steele, Gitelson & Rundquist in American Journal of Enology and Viticulture (2008) 59(3) Copyright © 2008, American Society for Enology and Viticulture. Used by permission.

Abstract

Leaf chlorophyll content provides valuable information about the physiological status of plants, and there is a need for accurate, efficient, practical methodologies to estimate this biophysical parameter. Reflectance measurement is a means of quickly and nondestructively assessing, in situ, the chlorophyll content in leaves. The objective of this study was to develop a precise, efficient, nondestructive technique to estimate leaf total chlorophyll (Chl) content in grapes. A relationship was established between Chl content and the red-edge chlorophyll index, based on reflectances in the red-edge (710–720 nm) and near-infrared (755–765 nm) spectral ranges, and the algorithm for Chl retrieval was calibrated. The accuracy of Chl prediction using an independent data set, containing sampled leaves from three field-grown grape cultivars (Edelweiss, Saint Croix, and DeChaunac), was evaluated with no re-parameterization (adjustment of the coefficients) after initial calibration. Although Chl in the validation data set was widely variable, from 3 to 506 mg m-2, the calibrated algorithm was capable of accurately predicting grape leaf Chl with RMSE <30 mg m-2. Such an approach has potential for developing simple hand-held field instrumentation for accurate nondestructive Chl estimation and in analyzing digital airborne or satellite imagery to assist in vineyard management decision making.

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