Electrophoresis: How scientists observe fragments of DNA

Authors

• Donald J. Lee, University of Nebraska - LincolnFollow
• Patricia Hain, University of Nebraska - Lincoln

Document Type

Learning Object

Date of this Version

2002

Comments

Published 2002 Plant and Soil Sciences eLibrary. Used by permission.

Abstract

Overview

This lesson describes gel electrophoresis and how the method is used in molecular genetic analysis.

Learning Objectives

This lesson describes gel electrophoresis and how the method is used in molecular genetic analysis. At the completion of this lesson, a learner should be able to:

• Define the terms “electrophoresis gel”, “electrophoresis buffer“, “ethidium bromide”, “fragment banding pattern”, “agarose” and “acrylamide”
• List the steps in the electrophoresis method and their proper order.
• Describe the basic principles and techniques involved in the use of electrophoresis to detect specific molecules and why the technique requires the geneticist to obtain or generate many copies of the molecules being detected.
• Contrast the electrophoresis methods used to detect DNA, RNA and proteins.
• Predict how DNA analysis techniques including electrophoresis procedures would need to be changed in order to visualize specific differences between DNA samples.
• Determine the genotype implied at a genetic locus by the pattern of banding observed in an electrophoresis gel.
• Predict the electrophoresis banding pattern observed in DNA samples based on the DNA segment differences in those DNA samples.

Modules

1. Overview
2. Learning Objectives
3. Electrophoresis Introduction
4. Electrophoresis gels: the race track
5. Current is the key
6. Preparing a gel
7. Pouring a gel
8. Loading a gel established the starting line
9. Conducting a fair race
10. The agarose obstacle course
11. The race in action
12. A photo finish
13. Fragment patterns and molecular genotypes
14. Alternative gels
15. RNA and Protein electrophoresis
16. Summary