Phenotypic and Genotypic Characterization of Uromyces appendiculatus from Phaseolus vulgaris in the Americas

Authors

C. M. Araya, University of Nebraska-Lincoln
A. T. Alleyne, University of Nebraska-Lincoln
J. R. Steadman, University of Nebraska-LincolnFollow
Kent M. Eskridge, University of Nebraska-LincolnFollow
D. P. Coyne, University of Nebraska-Lincoln

ORCID IDs

Kent M. Eskridge

Document Type

Article

Date of this Version

2004

Comments

Published in Plant Disease Vol. 88 No. 8, 2004. © 2004 The American Phytopathological Society. Used by permission.

Abstract

Populations of 90 Uromyces appendiculatus isolates were collected from throughout the Americas and evaluated for virulence on 19 standard bean rust differentials, and also on 12 landraces of Phaseolus vulgaris from South and Central America. The landrace differentials represented geographical centers of bean domestication. Three groups were observed. Two groups were isolates from centers of bean domestication and a third heterogeneous group comprised isolates from countries in South and Central America. Molecular analysis using random amplified polymorphic DNA–polymerase chain reaction (RAPD-PCR) was also conducted on these isolates. Cluster analysis of the molecular profiles showed three groups that corresponded to those obtained by virulence tests. These results show a clear differentiation of the pathogen population along similar lines as its host and suggest parallel evolution in the bean rust pathosystem.