The Internal Transcribed Spacer Region of Belonolaimus (Nemata: Belonolaimidae)

Authors

T. Cherry, Lincoln High School, Lincoln, NE
A. L. Szalanski, University of Nebraska-Lincoln
T. C. Todd, Kansas State University
Thomas O. Powers, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

1997

Comments

Published in Journal of Nematology 29 (1) :23-29. 1997. © The Society of Nematologists 1997.

Abstract

Belonolaimus isolates from six U.S. states were compared by restriction endonuclease digestion of amplified first internal transcribed spacer region (ITS1) of the nuclear ribosomal genes. Seven restriction enzymes were selected for evaluation based on restriction sites inferred from the nucleotide sequence of a South Carolina Belonolaimus isolate. Amplified product size from individuals of each isolate was approximately 700 bp. All Midwestern isolates gave distinct restriction digestion patterns. Isolates identified morphologically as Belonolaimus longicaudatus from Florida, South Carolina, and Palm Springs, California, were identical for ITS1 restriction patterns. The correlation between ITS1 restriction patterns and the distribution of B. longicaudatus isolates suggest that the California isolate is a relatively recent introduction into the state.