Plant Pathology, Department of

 

Department of Plant Pathology: Faculty Publications

Accessibility Remediation

If you are unable to use this item in its current form due to accessibility barriers, you may request remediation through our remediation request form.

ORCID IDs

James R. Alfano

Document Type

Article

Date of this Version

2004

Citation

Molecular Plant-Microbe Interactions Vol. 17, No. 5, 2004, pp. 447–455. Publication no. M-2004-0226-01R.

Comments

Copyright © 2004 The American Phytopathological Society. Used by permission.

Abstract

Type III secretion systems are highly conserved among gram-negative plant and animal pathogenic bacteria. Through the type III secretion system, bacteria inject a number of virulence proteins into the host cells. Analysis of the whole genome sequence of Pseudomonas syringae pv. tomato DC3000 strain identified a locus, named HopPtoF, that is homologous to the avirulence gene locus avrPphF in P. syringae pv. phaseolicola. The HopPtoF locus harbors two genes, ShcFPto and HopFPto, that are preceded by a single hrp box promoter. We present evidence here to show that ShcFPto and HopFPto encode a type III chaperone and a cognate effector, respectively. ShcFPto interacts with and stabilizes the HopFPto protein in the bacterial cell. Translation of HopFPto starts at a rare initiation codon ATA that limits the synthesis of the HopFPto protein to a low level in bacterial cells.

Share

COinS