Bioinformatics-Enabled Identification of the HrpL Regulon and Type III Secretion System Effector Proteins of Pseudomonas syringae pv. phaseolicola 1448A

Authors

Monica Vencato, Cornell UniversityFollow
Fang Tian, University of Nebraska-Lincoln
James R. Alfano, University of Nebraska-LincolnFollow
C. Robin Buell, Institute for Genomic ResearchFollow
Samuel Cartinhour, USDA-ARS
Genevieve A. DeClerck, USDA-ARS
David S. Guttman, University of TorontoFollow
John Stavrinides, University of TorontoFollow
Vinita Joardar, The Institute for Genomic Research
Magdalen Lindeberg, Cornell UniversityFollow
Philip A. Bronstein, USDA-ARS
John W. Mansfield, Imperial College at WyeFollow
Christopher R. Myers, Cornell UniversityFollow
Alan Collmer, Cornell UniversityFollow
David J. Schneider, USDA-ARSFollow

ORCID IDs

James R. Alfano

Document Type

Article

Date of this Version

2006

Citation

MPMI Vol. 19, No. 11, 2006, pp. 1193–1206. DOI: 10.1094/MPMI -19-1193.

Comments

U. S. government work.

Abstract

The ability of Pseudomonas syringae pv. phaseolicola to cause halo blight of bean is dependent on its ability to translocate effector proteins into host cells via the hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS). To identify genes encoding type III effectors and other potential virulence factors that are regulated by the HrpL alternative sigma factor, we used a hidden Markov model, weight matrix model, and type III targeting-associated patterns to search the genome of P. syringae pv. phaseolicola 1448A, which recently was sequenced to completion. We identified 44 high-probability putative Hrp promoters upstream of genes encoding the core T3SS machinery, 27 candidate effectors and related T3SS substrates, and 10 factors unrelated to the Hrp system. The expression of 13 of these candidate HrpL regulon genes was analyzed by real-time polymerase chain reaction, and all were found to be upregulated by HrpL. Six of the candidate type III effectors were assayed for T3SSdependent translocation into plant cells using the Bordetella pertussis calmodulin-dependent adenylate cyclase (Cya) translocation reporter, and all were translocated. PSPPH1855 (ApbE-family protein) and PSPPH3759 (alcohol dehydrogenase) have no apparent T3SS-related function; however, they do have homologs in the model strain P. syringae pv. tomato DC3000 (PSPTO2105 and PSPTO0834, respectively) that are similarly upregulated by HrpL. Mutations were constructed in the DC3000 homologs and found to reduce bacterial growth in host Arabidopsis leaves. These results establish the utility of the bioinformatic or candidate gene approach to identifying effectors and other genes relevant to pathogenesis in P. syringae genomes.