Sequence variation in two genes determines the efficacy of transmission of citrus tristeza virus by the brown citrus aphid

Authors

S. J. Harper, University of FloridaFollow
N. Killiny, University of FloridaFollow
Satyanarayana Tatineni, USDA-ARSFollow
S. Gowda, University of FloridaFollow
S. J. Cowell, University of Florida
T. Shilts, University of Florida
W. O. Dawson, University of FloridaFollow

Document Type

Article

Date of this Version

2016

Citation

Arch Virol (2016) 161:3555–3559, DOI 10.1007/s00705-016-3070-x

Comments

U.S. government work.

Abstract

Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in to the poorly transmissible T36-based infectious clone. We found that introducing p65 or p61 sequences from FS577 significantly increased transmission efficacy. Interestingly, replacement of both genes produced a greater increase than either gene alone, suggesting that CTV transmission requires the concerted action of co-evolved p65 and p61 proteins.