The complete nucleotide sequence and genome organization of the M RNA segment of peanut bud necrosis tospovirus and comparison with other tospoviruses

Authors

Satyanarayana Tatineni, USDA-ARSFollow
S. E. Mitchell, USDA-ARS
D. V. R. Reddy, International Crops Research Institute for the Semi-Arid Tropics
S. Kresovich, USDA-ARS
R. Jarret, USDA-ARS
R. A. Naidu, lInternational Crops Research Institute for the Semi-Arid Tropics
S. Gowda, University of Florida
J. W. Demski, University of Georgia

Document Type

Article

Date of this Version

1996

Citation

Journal of General Virology (1996), 77, pp. 2347-2352.

Comments

U.S. government work.

Abstract

The M RNA of peanut bud necrosis virus (PBNV; synonym groundnut bud necrosis virus) is 4801 nucleotides in length. It comprised two ORFs in an ambisense organization and terminal inverted repeats. The 3' large ORF (3363 nucleotides in the virus-complementary strand) encoded a protein with a predicted size of 127.2 kDa which was identified as the glycoprotein precursor (GP) of the G1 and G2 glycoproteins. A comparison of the deduced amino acid sequence of GP revealed 37 % identity and 58-59% similarity with that of tomato spotted wilt virus (TSWV, serogroup I) and impatiens necrotic spot virus (INSV, serogroup III), and 21-23% identity and 44--47 % similarity with those of other members of the genus Bunyavirus. The 5' small ORF (924 nucleotides in the virus-sense strand) encoded a 34.2 kDa protein which was identified as the non-structural (NSm) protein based on 41-43% identity and 60-63% similarity with that of TSWV and INSV. Defective RNA molecules derived from the genomic M RNA were detected during continuous passage of the virus by sap inoculations.