Plant Pathology Department

 

ORCID IDs

0000-0002-5052-1782

Document Type

Article

Date of this Version

2018

Citation

NATURE COMMUNICATIONS | (2018) 9:1552

Comments

© The Author(s) 2018

Open access

DOI: 10.1038/s41467-018-04051-9

Abstract

The Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a “block-based” reconstruction method for extending the resolution limit. We find the Ewald sphere effect limits the resolution of large objects, especially large viruses. After processing two real datasets of large viruses, we show that our procedure can extend the resolution for both datasets and can accommodate the flexibility associated with large protein complexes.

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