Plant Pathology, Department of
Department of Plant Pathology: Faculty Publications
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Document Type
Article
Date of this Version
February 2004
Abstract
The RNA genome (9346 nucleotides) of Oat necrotic mottle virus (ONMV) was cloned and sequenced. Complete genome comparisons indicated that ONMV, currently classified as a rymovirus, was most closely related (~73% nucleotide, ~79% amino acid identity) to the tritimovirus Wheat streak mosaic virus (WSMV). ONMV encoded a single polyprotein, with proteinase cleavage sites very similar to those ofWSMV. Pairwise comparison of ONMV andWSMV cistrons revealed that P3 was most conserved (~79% nucleotide, ~86% amino acid), whereas HC-Pro was most divergent (~67% nucleotide, ~69% amino acid). In contrast, the ONMV sequence was distantly related (~40% nt, ~26% amino acid identity) to that of the rymovirus Ryegrass mosaic virus, with highest sequence conservation noted within the NIb cistron (~47% nucleotide, ~41% amino acid identity). These results firmly establish that ONMV is not a rymovirus but is instead a distinct species of the genus Tritimovirus.
Comments
Published in Arch Virol (2004) 149: 633–640.