United States Patent Application Publication: COUNTER SELECTION STRATEGY FOR GRAM-NEGATIVE BACTERIA

Authors

Stephen K. Farrand, Seymour, ILFollow
Paul E. Staswick, University of Nebraska - LincolnFollow
Thomas E. Clemente, University of Nebraska - LincolnFollow

Document Type

Article

Date of this Version

May 2002

Comments

United States Patent Application Publication NO.: US 2002/0061579 A1.
Pub. Date: May 23,2002

Abstract

A Gram-negative bacterium useful for genetically engineering plants is provided. The Gram-negative bacterium contains, as part of genome, an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Alternatively, the Gram-negative bacterium comprises a recombinant nucleic acid construct containing an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Also provided are recombinant nucleic acid constructs comprising an inducible regulatory sequence operatively coupled to a nucleotide sequence encoding a levansucrase and a method for transforming plants using the Gram-negative bacterium of the present invention.