A preliminary linkage map of the tick, Ixodes scapularis

Authors

• Amy J. Ullmann, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, P.O. Box 2087, Rampart Road, Foothills Campus, Fort Collins, CO 80522, USA
• Joseph Piesman, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, P.O. Box 2087, Rampart Road, Foothills Campus, Fort Collins, CO 80522, USA
• M.C. Dolan, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, P.O. Box 2087, Rampart Road, Foothills Campus, Fort Collins, CO 80522, USA
• William C. Black, IV, Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1677, USA

Date of this Version

2002

Comments

Published in Experimental and Applied Acarology 28: 107–126, 2002.

Abstract

A linkage map of the Ixodes scapularis genome was constructed based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F₁ intercross progeny from a single, field-collected P₁ female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of ∼10⁹ bp, the relationship of physical to genetic distance is ∼300 kb/cM in the I. scapularis genome.