A preliminary linkage map of the tick, Ixodes scapularis

Authors

Amy J. Ullmann, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, P.O. Box 2087, Rampart Road, Foothills Campus, Fort Collins, CO 80522, USA
Joseph Piesman, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, P.O. Box 2087, Rampart Road, Foothills Campus, Fort Collins, CO 80522, USA
M.C. Dolan, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, P.O. Box 2087, Rampart Road, Foothills Campus, Fort Collins, CO 80522, USA
William C. Black, IV, Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1677, USA

Date of this Version

2002

Comments

Published in Experimental and Applied Acarology 28: 107–126, 2002.

Abstract

A linkage map of the Ixodes scapularis genome was constructed based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F₁ intercross progeny from a single, field-collected P₁ female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of ∼10⁹ bp, the relationship of physical to genetic distance is ∼300 kb/cM in the I. scapularis genome.