Rapid identification and typing of Mycobacterium avium complex using Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) library and biomarker based approaches.

Authors

Claudia AntonikaFollow
John Dustin Loy, SVBMS University of Nebraska LincolnFollow
Raul Barletta, SVBMS University of Nebraska LincolnFollow
Michael Collins, Department of Pathobiology University of Wisconsin MadisonFollow

Date of this Version

Spring 3-20-2018

Document Type

Poster

Citation

Rathnaiah, G., Zinniel, D. K., Bannantine, J. P., Stabel, J. R., Gröhn, Y. T., Collins, M. T., & Barletta, R. G. (2017). Pathogenesis, Molecular Genetics, and Genomics of Mycobacterium avium subsp. paratuberculosis, the Etiologic Agent of Johne's Disease. Frontiers in veterinary science, 4, 187. doi:10.3389/fvets.2017.00187 2. Shin, S. J., Lee, B. S., Koh, W. J., Manning, E. J., Anklam, K., Sreevatsan, S., … Collins, M. T. (2010). Efficient differentiation of Mycobacterium avium complex species and subspecies by use of fivetarget multiplex PCR. Journal of clinical microbiology, 48(11), 4057– 4062. doi:10.1128/JCM.00904-10

Comments

the project will be continued using different instrument

Abstract

Bacteria associated with the Mycobacterium avium complex (MAC) cause significant problems in animal agriculture and cause large economic losses and morbidity of livestock. Two clinically important subspecies of M. avium are M.avium ssp paratuberculosis (MAP) and M. avium ssp hominissuis (MAH). MAP is the etiologic agent of Johne’s disease, a chronic and fatal enteritis in ruminants, that has been linked to Crohn’s disease in humans.1 MAH has zoonotic potential as it is one of the leading causes of secondary infections in AIDS patients. Identification of M. avium in clinical samples is challenging as they have overlapping host ranges and clinical signs. Information about the restriction of host specificity of M.avium also have not been determined. Available diagnostic tests for M. avium, such as blood and biochemical tests are labor intensive, time consuming, and lack sensitivity. Advanced methods such as matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) could provide rapid, low-cost, and more accurate diagnostic test to differentiate MAP which can be implemented into diagnostic workflows in veterinary diagnostic labs. The purpose of this project is to differentiate using M. avium from field isolates, particularly MAP and MAH and other members of MAC using a library based approach, and to examine the host specificity and tropism of isolates using these methods.