Rapid and Simultaneous Detection of bla_KPC and bla_NDM by Use of Multiplex Real-Time PCR

Authors

Michael Milillo, Walter Reed Army Institute of Research
Yoon I. Kwak, Walter Reed Army Institute of Research
Paige E. Waterman, Walter Reed Army Institute of Research
Emil Lesho, Walter Reed Army Institute of Research
Patrick McGann, Walter Reed Army Institute of ResearchFollow

Document Type

Article

Date of this Version

2013

Citation

Published in Journal of Clinical Microbiology (2013) 51(4): 1247-1249. doi:10.1128/JCM.03316-12

Abstract

The increasing incidence of carbapenem nonsusceptibility among clinically important species is of global concern. Identification of the molecular mechanisms underlying carbapenem nonsusceptibility is critical for epidemiological investigations. In this report, we describe a real-time PCR-based assay capable of simultaneously detecting bla_KPC and bla_NDM, two of the most important carbapenemases, directly from culture in less than 90 min. The assay was validated with bla_KPC- and bla_NDM-carrying clinical isolates and demonstrated 100% concordance with the Carba NP test.