Detection of New Delhi Metallo-β-Lactamase (Encoded by bla_NDM-1) in Acinetobacter schindleri during Routine Surveillance

Authors

• Patrick McGann, Walter Reed Army Institute of ResearchFollow
• Michael Milillo, Walter Reed Army Institute of Research
• Robert J. Clifford, Walter Reed Army Institute of Research
• Erik Snesrud, Walter Reed Army Institute of Research
• Lindsay Stevenson, Walter Reed National Military Medical Center
• Michael G. Backlund, Walter Reed National Military Medical Center
• Helen B. Viscount, Walter Reed National Military Medical Center
• Reyes Quintero, Walter Reed Army Institute of Research
• Yoon I. Kwak, Walter Reed Army Institute of Research
• Michael J. Zapor, Walter Reed National Military Medical Center
• Paige W. Waterman, Walter Reed Army Institute of Research
• Emil P. Lesho, Walter Reed Army Institute of Research

Document Type

Article

Date of this Version

2013

Citation

Published in Journal of Clinical Microbiology (2013) 51(6): 1942-1944. DOI:10.1128/JCM.00281-13

Abstract

A carbapenem-resistant Alcaligenes faecalis strain was isolated from a surveillance swab of a service member injured in Afghanistan. The isolate was positive for bla_NDM by real-time PCR. Species identification was reevaluated on three identification systems but was inconclusive. Genome sequencing indicated that the closest relative was Acinetobacter schindleri and that bla_NDM-1 was carried on a plasmid that shared >99% identity with one identified in an Acinetobacter lwoffii isolate. The isolate also carried a novel chromosomally encoded class D oxacillinase.