A phage-displayed peptide recognizing porcine aminopeptidase N is a potent small molecule inhibitor of PEDV entry

Authors

Fandan Meng, Northeast Agricultural University
Siqingaowa Suo, Northeast Agricultural University
Dante S. Zarlenga, Agricultural Research Service
Yingying Cong, Northeast Agricultural University
Xiaowei Ma, Northeast Agricultural University
Qiong Zhao, Northeast Agricultural University
Xiaofeng Ren, Northeast Agricultural UniversityFollow

Date of this Version

3-25-2014

Citation

2014 Published by Elsevier Inc.

Comments

http://dx.doi.org/10.1016/j.virol.2014.01.010

Abstract

Three phage-displayed peptides designated H, S and F that recognize porcine aminopeptidase N (pAPN), the cellular receptor of porcine transmissible gastroenteritis virus (TGEV) were able to inhibit cell infection by TGEV. These same peptides had no inhibitory effects on infection of Vero cells by porcine epidemic diarrhea virus (PEDV). However, when PEDV, TGEV and porcine pseudorabies virus were incubated with peptide H (HVTTTFAPPPPR), only infection of Vero cells by PEDV was inhibited. Immunofluorescence assays indicated that inhibition of PEDV infection by peptide H was independent of pAPN. Western blots demonstrated that peptide H interacted with PEDV spike protein and that pre-treatment of PEDV with peptide H led to a higher inhibition than synchronous incubation with cells. These results indicate direct interaction with the virus is necessary to inhibit infectivity. Temperature shift assays demonstrated that peptide H inhibited pre-attachment of the virus to the cells.