US Fish & Wildlife Service


Date of this Version



Published in Transactions of the American Microscopical Society, Vol. 73, No. 3 (Jul., 1954), pp. 328-329


Following the method of Brooke and Goldman (1949), the author was able to fix, mount and stain small helminths and parasitic protozoa of fish in polyvinyl alcohol-fixative smears. However, because there was much granular material in the PVA-Schaudin's mixture, AFA fixative was substituted for the Schaudin's. The PVA went into solution readily using this fixative. The solution was accomplished by adding 5 grams Elvanol #71-301 to 62 ml. of water, heating to 950 C. until dissolved and adding 5 ml. of acetic acid, 1-1/2 ml. of glycerine, 10 ml. of formaldehyde, and 30 ml. of 95 percent alcohol.