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Crescent Lake National Wildlife Refuge (NWR) is located in the Sandhills of western Nebraska, an area which contains the largest remaining tract of mid-grass and tall-grass prairie in North America. Land use surrounding the refuge is dominated by rangeland and land use practices likely result in minimal inputs of point or non-point source pollution. Industrial development in the area is minimal and the refuge complex is believed to be in fairly pristine condition. The use of persistent organochlorine pesticides on the refuge has been limited. The objectives of this study were to determine background concentrations of metals in biotic and abiotic components of the refuge and document these results to utilize as baseline information for reference and future contaminants investigations.