Chloroviruses N-linked glycans share a new type of conserved core architecture unprecedented in any form of life / [Published as] N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent

Authors

• Cristina De Castro, University of NapoliFollow
• Immacolata Speciale, University of Napoli
• Garry Duncan, Nebraska Wesleyan UniversityFollow
• David Dunigan, University of Nebraska - LincolnFollow
• Irina Agarkova, University of Nebraska-LincolnFollow
• Rosa Lanzetta, University of Napoli
• Luisa Sturiale, Consiglio Nazionale delle Ricerche–Istituto di Chimica e Tecnologia dei Polimeri
• Angelo Palmigiano, Consiglio Nazionale delle Ricerche–Istituto di Chimica e Tecnologia dei Polimeri
• Domenico Garozzo, Consiglio Nazionale delle Ricerche–Istituto di Chimica e Tecnologia dei Polimeri
• Antonio Molinaro, University of Napoli
• Michela Tonetti, University of GenovaFollow
• James L. Van Etten, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

1-2016

Citation

Published in final edited form as: Angew Chem Int Ed Engl. 2016 January 11; 55(2): 654–658.

doi:10.1002/anie.201509150.

Author manuscript; available in PMC 2017 January 11.

Comments

Copyright 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Used by permission.

Abstract

N-glycosylation is a fundamental modification of proteins that exists in the three domains of life and in some viruses, including the chloroviruses, for which a new type of core N-glycan is described. This N-glycan core structure common to all chloroviruses is a pentasaccharide with a β-glucose linked to an asparagine residue that is not located in the typical sequon N-X-T/S. The glucose is linked to a terminal xylose unit and a hyperbranched fucose, in turn substituted with a terminal galactose and a second xylose residue. The third position of the fucose unit is always linked to a rhamnose, which is a semi-conserved element because its absolute configuration is virus-dependent. Additional decorations occur on this core N-glycan and represent a molecular signature for each chlorovirus.

Includes supplemental materials.