Sequence and annotation of the 288-kb ATCV-1 virus that infects an endosymbiotic chlorella strain of the heliozoon Acanthocystis turfacea

Authors

• Lisa A. Fitzgerald, U.S. Naval Research Laboratory, Washington, DCFollow
• Michael V. Graves, University of Massachusetts–LowellFollow
• Xiao Li, University of Massachusetts-Lowell
• James Hartigan, Agencourt Bioscience Corporation, Beverly, MA
• Artur J.P. Pfitzner, University of HohenheimFollow
• Ella Hoffart, University of Hohenheim
• James L. Van Etten, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

2007

Citation

Published in final edited form as: Virology. 2007 June 5; 362(2): 350–361.

DOI: 10.1016/j.virol.2006.12.028

PMID:

17276475

PMCID:

PMC2018652

Comments

Published by Elsevier. Used by permission.

Abstract

Acanthocystis turfacea chlorella virus (ATCV-1), a prospective member of the family Phycodnaviridae, genus Chlorovirus, infects a unicellular, eukaryotic, chlorella-like green alga, Chlorella SAG 3.83, that is a symbiont in the heliozoon A. turfacea. The 288,047-bp ATCV-1 genome is the first virus to be sequenced that infects Chlorella SAG 3.83. ATCV-1 contains 329 putative protein-encoding and 11 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands and intergenic space is minimal. Thirty-four percent of the viral gene products resemble entries in the public databases, including some that are unexpected for a virus. For example, these unique gene products include ribonucleoside-triphosphate reductase, dTDP-D-glucose 4,6 dehydratase, potassium ion transporter, aquaglyceroporin, and mucindesulfating sulfatase. Comparison of ATCV-1 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that about 80% of the ATCV-1 genes are present in PBCV-1.