Sequence and annotation of the 288-kb ATCV-1 virus that infects an endosymbiotic chlorella strain of the heliozoon Acanthocystis turfacea

Authors

Lisa A. Fitzgerald, U.S. Naval Research Laboratory, Washington, DCFollow
Michael V. Graves, University of Massachusetts–LowellFollow
Xiao Li, University of Massachusetts-Lowell
James Hartigan, Agencourt Bioscience Corporation, Beverly, MA
Artur J.P. Pfitzner, University of HohenheimFollow
Ella Hoffart, University of Hohenheim
James L. Van Etten, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

2007

Citation

Published in final edited form as: Virology. 2007 June 5; 362(2): 350–361.

DOI: 10.1016/j.virol.2006.12.028

PMID:

17276475

PMCID:

PMC2018652

Comments

Published by Elsevier. Used by permission.

Abstract

Acanthocystis turfacea chlorella virus (ATCV-1), a prospective member of the family Phycodnaviridae, genus Chlorovirus, infects a unicellular, eukaryotic, chlorella-like green alga, Chlorella SAG 3.83, that is a symbiont in the heliozoon A. turfacea. The 288,047-bp ATCV-1 genome is the first virus to be sequenced that infects Chlorella SAG 3.83. ATCV-1 contains 329 putative protein-encoding and 11 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands and intergenic space is minimal. Thirty-four percent of the viral gene products resemble entries in the public databases, including some that are unexpected for a virus. For example, these unique gene products include ribonucleoside-triphosphate reductase, dTDP-D-glucose 4,6 dehydratase, potassium ion transporter, aquaglyceroporin, and mucindesulfating sulfatase. Comparison of ATCV-1 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that about 80% of the ATCV-1 genes are present in PBCV-1.