Veterinary and Biomedical Sciences, Department of

 

Date of this Version

11-2017

Citation

Porcine Health Management 3:22 (2017), pp. 1–12.

doi: 10.1186/s40813-017-0068-z

Comments

Copyright © 2017 Sarah Vitosh-Sillman, John Dustin Loy, Bruce Brodersen, Kent Eskridge, and Amy Millmier Schmidt. Open access licensed.

Abstract

Porcine epidemic diarrhea virus (PEDV) is an enteric disease of swine that has emerged as a worldwide threat to swine herd health and production. Substantial research has been conducted to assess viability of the virus on surfaces of vehicles and equipment, in feed and water, and on production building surfaces, but little is known about the persistence in PEDV-infected carcasses and effective disposal methods thereof. This study was conducted to quantify the persistence of PEDV RNA via quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) at various time-temperature combinations and in infected piglet carcasses subjected to composting. Although this method does not distinguish between infectious and noninfectious virus, it is a rapid and sensitive test to evaluate materials for evidence of virus genome.

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