Comparison of the Direct Agglutination and Indirect Hemagglutination Tests in the Determination of Blood Serum Titers to Escherichia coli Organisms

Authors

• I. A. Schipper, North Dakota State University, Fargo
• Clayton L. Kelling, University of Nebraska-LincolnFollow
• H. Ebeltoft, North Dakota State University, Fargo
• D. Graves, North Dakota State University, Fargo

Document Type

Article

Date of this Version

March 1973

Comments

Published in APPLIED MICROBIOLOGY, Mar. 1973, p. 458-460, Vol. 25, No. 3. Copyright © 1973 American Society for Microbiology. Used by permission.

Abstract

A comparison of the direct agglutination test and the indirect hemagglutination test for the detection of blood serum antibodies to Escherichia coli organisms indicated that these serological tests were comparable. In some instances the indirect hemagglutination test provided higher endpoint readings. Preparation of the antigens for the indirect hemagglutination test was more time consuming than for the direct agglutination test. Crude extract and purified polysaccharides were comparable as red blood cell sensitizing agents.