Ornithine Decarboxylase Encoded by Chlorella Virus PBCV-1

Authors

• Tiara A. Morehead, University of Nebraska-Lincoln
• James R. Gurnon, University of Nebraska-LincolnFollow
• Byron Adams, University of FloridaFollow
• Kenneth Nickerson, University of Nebraska-LincolnFollow
• Lisa A. Fitzgerald, University of Nebraska-LincolnFollow
• James L. Van Etten, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

5-12-2002

Comments

Published in Virology 301 (2002), pp. 165-175. doi:10.1006/viro.2002.1573 Copyright © 2002 Elsevier Science (USA). Used by permission.

Abstract

Sequence analysis of the 330-kb genome of chlorella virus PBCV-1 revealed an open reading frame, A207R, which encodes a protein with 37–41% amino acid identity to ornithine decarboxylase (ODC) from many eukaryotic organisms. The a207r gene was cloned and the protein was expressed as a His-A207R fusion protein in Escherichia coli. The recombinant protein catalyzes pyridoxal 5’-phosphate-dependent decarboxylation of ornithine to putrescine, the first step in the polyamine biosynthetic pathway The enzyme has a pH optimum of 9.0 and a temperature optimum of 42°C, and it requires dithiothreitol for maximal activity. The enzyme has a Km, for ornithine of 0.78 mM and a specific activity of 100 μmol/min/mg protein. PBCV-1 ODC is quite sensitive to the competitive inhibitor L-arginine and the irreversible inhibitor difluoromethylarginine but it is less sensitive to the irreversible inhibitor difluoromethylornithine. The a207r gene is expressed both early and late in PBCV-1 infection and is highly conserved among the chlorella viruses. The 42-kDa PBCV-1 ODC (372 amino acids) is the smallest ODC in the databases and, to our knowledge, is the first virus-encoded ODC.