Enhanced Competitiveness of Tomato Bushy Stunt Virus Defective Interfering RNAs by Segment Duplication or Nucleotide Insertion

Authors

K. Andrew White, University of Nebraska-Lincoln
Thomas Jack Morris, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

9-1-1994

Comments

Published in JOURNAL OF VIROLOGY, Sept. 1994, p. 6092-6096 Vol. 68, No. 9 0022-538X/94/$04.00+0 Copyright © 1994, American Society for Microbiology. Used by permission.

Abstract

We have analyzed atypical tomato bushy stunt virus defective interfering (DI) RNA species which accumulated during a passage series in protoplasts. We present a rationale for the order of appearance of these molecules and show, using competition assays, that either segment duplication or single nucleotide insertion can enhance DI RNA competitiveness. Possible mechanisms for the introduction of the modifications observed in these DI RNAs are discussed.