Virology, Nebraska Center for


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Published in JOURNAL OF VIROLOGY, Sept. 1994, p. 6092-6096 Vol. 68, No. 9 0022-538X/94/$04.00+0 Copyright © 1994, American Society for Microbiology. Used by permission.


We have analyzed atypical tomato bushy stunt virus defective interfering (DI) RNA species which accumulated during a passage series in protoplasts. We present a rationale for the order of appearance of these molecules and show, using competition assays, that either segment duplication or single nucleotide insertion can enhance DI RNA competitiveness. Possible mechanisms for the introduction of the modifications observed in these DI RNAs are discussed.

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