Processing and Localization of Dengue Virus Type 2 Polyprotein Precursor NS3-NS4A-NS4B-NS5

Authors

Luwen Zhang, University of Nebraska-LincolnFollow
P. Maruthi Mohan, University of Kansas Medical Center
R. Padmanabhan, University of Kansas Medical Center

Document Type

Article

Date of this Version

12-1-1992

Comments

Published in JOURNAL OF VIROLOGY, Dec. 1992, p. 7549-7554, Vol. 66, No. 12. Copyright © 1992, American Society for Microbiology. Used by permission.

Abstract

Processing of dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5 could be mediated by the catalytically active NS3 protease domain and NS2B in trans at the dibasic sites NS3-NS4A and NS4B-NS5. Subcellular localization of the unprocessed precursor NS3-NS4A-NS4B-NS5 showed that it was confined to a distinct subcellular organelle in the cytoplasm, which was distinct from the distribution of the mature NS5.