Construction and Characterization of a Chimeric Virus (BIV/HIV-1) Carrying the Bovine Immunodeficiency Virus gag-pol Gene: Research Letters

Authors

• Guomin Chen, Institute of Virology, Beijing
• Shuhui Wang, Nankai University
• Kun Xiong, Nankai University
• Jinzhong Wang, Nankai University
• Tao Ye, Institute of Virology, Beijing
• Wenping Dong, Institute of Virology, Beijing
• Qi Wang, Institute of Virology, Beijing
• Qimin Chen, Nankai University
• Yunqi Geng, Nankai University
• Charles Wood, University of Nebraska-LincolnFollow
• Yi Zeng, Institute of Virology, Beijing

Document Type

Article

Date of this Version

1-4-2002

Comments

Published in AIDS 16(1) (January 4, 2002): 123-125. Copyright 2002, Lippincott Williams & Wilkins. Used by permission.

Abstract

HIV-1HXB2 5′LTR region, most of BIVR29 gag-pol segment and HIV-1HXB2 pol IN-3′LTR region were respectively amplified. A chimeric clone, designated as pHBIV₃₇₅₃, was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV₃₇₅₃. The replication and expressions of the chimeric virus (HBIV₃₇₅₃) were monitored by RT activity and IFA. The results firstly demonstrated that it is possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol gene.