Construction and Characterization of a Chimeric Virus (BIV/HIV-1) Carrying the Bovine Immunodeficiency Virus gag-pol Gene: Research Letters

Authors

Guomin Chen, Institute of Virology, Beijing
Shuhui Wang, Nankai University
Kun Xiong, Nankai University
Jinzhong Wang, Nankai University
Tao Ye, Institute of Virology, Beijing
Wenping Dong, Institute of Virology, Beijing
Qi Wang, Institute of Virology, Beijing
Qimin Chen, Nankai University
Yunqi Geng, Nankai University
Charles Wood, University of Nebraska-LincolnFollow
Yi Zeng, Institute of Virology, Beijing

Document Type

Article

Date of this Version

1-4-2002

Comments

Published in AIDS 16(1) (January 4, 2002): 123-125. Copyright 2002, Lippincott Williams & Wilkins. Used by permission.

Abstract

HIV-1HXB2 5′LTR region, most of BIVR29 gag-pol segment and HIV-1HXB2 pol IN-3′LTR region were respectively amplified. A chimeric clone, designated as pHBIV₃₇₅₃, was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV₃₇₅₃. The replication and expressions of the chimeric virus (HBIV₃₇₅₃) were monitored by RT activity and IFA. The results firstly demonstrated that it is possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol gene.