Evaluation of monoclonal antibody–based immunohistochemistry for the detection of European and North American Porcine reproductive and respiratory syndrome virus and a comparison with in situ hybridization and reverse transcription polymerase chain reaction

Authors

• Kiwon Han, Seoul National University, Korea
• Hwi Won Seo, Seoul National University, Korea
• Yeonsu Oh, Seoul National University, Korea
• Ikjae Kang, Seoul National University, Korea
• Changhoon Park, Seoul National University, Korea
• Sang Hoon Kang, Kyunghee University, Seoul, Korea
• Sung-Hoon Kim, Kyunghee University, Seoul, Korea
• Bog-Hieu Lee, Chung-Ang University, Korea
• Byung Joon Kwon, University of Nebraska-LincolnFollow
• Chanhee Chae, Seoul National University, KoreaFollow

Document Type

Article

Date of this Version

2012

Citation

Journal of Veterinary Diagnostic Investigation 24:4 (2012), pp. 719–724; doi: 10.1177/1040638712446507

Comments

Copyright © 2012 Kiwon Han, Hwi Won Seo, Yeonsu Oh, Ikjae Kang, Changhoon Park, Sang Hoon Kang, Sung-Hoon Kim, Bog- Hieu Lee, Byungjoon Kwon, Chanhee Chae. Published by Sage Publications on behalf of American Association of Veterinary Laboratory Diagnosticians, Inc. Used by permission.

Abstract

The objective of the present study was to compare the ability of 2 monoclonal antibodies (mAbs; SDOW17 and SR30) to detect types 1 and 2 Porcine reproductive and respiratory syndrome virus (PRRSV) in formalin-fixed, paraffin-embedded (FFPE) lung tissues by immunohistochemistry (IHC) and to compare the immunohistochemical results with in situ hybridization (ISH) and reverse transcription nested polymerase chain reaction (RT-nPCR) detection techniques. Lungs from 30 experimentally infected pigs (15 pigs with each genotype of PRRSV) and 20 naturally infected pigs (10 pigs with each genotype of PRRSV) with types 1 and 2 PRRSV, respectively, were used for the IHC, ISH, and RTnPCR analyses. The SR30 mAb-based IHC detected significantly more type 1 PRRSV-positive cells in the accessory and caudal lobes from the experimentally infected pigs at 7 (P = 0.025) and 14 (P = 0.018) days postinoculation, respectively, compared to the SDOW17 mAb-based IHC. The results demonstrated that SR30 mAb-based IHC is useful for detecting both types 1 and 2 PRRSV antigen in FFPE lung tissues.