Characterization of Species C Human Adenovirus Serotype 6 (Ad6)

Authors

Eric A. Weaver, University of Nebraska-LincolnFollow
Reeti Khare, Mayo Graduate School, Rochester
Mathew L. Hillestad, Mayo Graduate School, Mayo Clinic
Donna Palmer, Baylor College of Medicine
Philip Ng, Baylor College of MedicineFollow
Michael A. Barry, Mayo Clinic, RochesterFollow

Document Type

Article

Date of this Version

3-30-2012

Citation

2012 Authors

doi:10.1016/j.virol.2010.10.041

Comments

Virology. 2011 March 30; 412(1): 19–27.

Abstract

Adenovirus serotype (Ad5) is the most studied Ad. Ad1, 2, and 6 are also members of species C Ad and are presumed to have biologies similar to Ad5. In this work, we have compared the ability of Ad1, 2, 5, and 6 to infect liver and muscle after intravenous and intramuscular injection. We found that Ad6 was surprisingly the most potent at liver gene delivery and that Ad1 and Ad2 were markedly weaker than Ad5 and 6. To understand these differences, we sequenced the Ad6 genome. This revealed that the Ad6 fiber protein is surprisingly three shaft repeats shorter than the others which may explain differences in virus infectivity in vitro, but not in the liver. Comparison of hexon hypervariable regions (HVRs) suggests that the higher transduction by Ad5 and 6 as compared to Ad1 and 2 may be related to differences in charge and length.