Effect of Milk on Fibronectin and Collagen Type I Binding to Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Mastitis

Authors

J. Miedzobrodzki, University of Lund
A. S. Naidu, University of Lund
J. L. Watts, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Homer, Louisiana
Pawel Ciborowski, University of Nebraska Medical CenterFollow
K. Palm, University of Lund

Document Type

Article

Date of this Version

3-1-1989

Comments

Published in JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1989, p. 540-544. 0095-1 137/89/030540-05$02.0010 Copyright 0 1989, American Society for Microbiology. Used by permission.

Abstract

Tryptic soy broth (TSB)-grown cells of Staphylococcus aureus isolated from acute and chronic bovine mastitis bound mainly ¹²⁵I-fibronectin (¹²⁵I-Fn), whereas strains of nine species of coagulase-negative staphylococci showed a predominant interaction with ¹²⁵I-collagen (¹²⁵I-Cn) type I. A particle agglutination assay (PAA) was used to examine the interaction of coagulase-negative staphylococci with ¹²⁵1-Fn and ¹²⁵I-Cn immobilized on latex. All 368 coagulase-negative staphylococci demonstrated high ¹²⁵I-Cn and moderate to low ¹²⁵I-Fn interactions in the PAA. Cn-PAA reactivity was high among strains of Staphylococcus xylosus (84.2%), Staphylococcus simulans (77.8%), Staphylococcus epidermidis (76.7%), and Staphylococcus hyicus (74.3%), whereas all six Staphylococcus capitis strains clumped Cn-PAA reagent. Incubating TSB-grown cells in 10% skim milk for 1 h decreased the ¹²⁵I-Fn- and ¹²⁵I-Cn-binding affinity in most of the S. aureus and coagulase-negative staphylococci, while growth in 10% skim milk for 18 h resulted in more than 90% decrease or complete loss of interaction with these proteins. Decreased 1251-Fn binding in the presence of milk was correlated with protease production but not with ¹²⁵I-Cn binding.