Comparison of Two Diagnostic Assays for the Detection of Serum Neutralizing Antibody to Porcine Epidemic Diarrhea Virus

Authors

Justin Brown, Iowa State University
Korakrit Poonsuk, University of Nebraska-Lincoln
Ting-Yu Cheng, Ohio State University
Chris Rademacher, Iowa State University
Erin Kalkwarf, Iowa State University
Liying Tian, Iowa State University
Lauren A. McKeen, Iowa State University
Chong Wang, Iowa State University
Luis Gimenez-Lirola, Iowa State University
Gimenez-Lirola Baum, Iowa State University
Locke A. Karriker, Iowa State University

Document Type

Article

Date of this Version

2-20-2023

Citation

Brown, J.; Poonsuk, K.; Cheng, T.-Y.; Rademacher, C.; Kalkwarf, E.; Tian, L.; McKeen, L.A.; Wang, C.; Gimenez-Lirola, L.; Baum, D.; et al. Comparison of Two Diagnostic Assays for the Detection of Serum Neutralizing Antibody to Porcine Epidemic Diarrhea Virus. Animals 2023, 13, 757. https:// doi.org/10.3390/ani13040757

Comments

Open access.

Abstract

Lactogenic immunity is important for the protection of piglets against many pathogens including porcine epidemic diarrhea virus. Circulating neutralizing antibodies levels in sow sera may help determine if a detectable immune response could confer protection to piglets. Neutralizing antibodies can be detected through various diagnostic assays. This study evaluated the diagnostic characteristics of two neutralizing antibody assays for porcine epidemic diarrhea virus neutralizing antibodies in serum of challenged gilts. Four treatment groups, control, non-vaccinated, vaccinated prior to challenge, and vaccinated following challenge, were comprised of 20 gilts. Serum sample were collected from each gilt prior to and following challenge with porcine epidemic diarrhea virus. Samples were evaluated for the presence of neutralizing antibodies via a fluorescent focus neutralization assay and a high-throughput neutralization assay. Diagnostic sensitivity and specificity for the fluorescent focus neutralization and high-throughput neutralization assays for this study were optimized at a cutoff of a dilution of 80 and 80% fluorescent reduction respectively and demonstrated moderate agreement based off the kappa statistic. The focus fluorescent neutralization and high-throughput neutralization assays can be used to monitor the status of neutralizing antibodies within animals or a population of animals. The high-throughput assay has advantages over the focus fluorescent assay in that it has a higher specificity at the indicated cut-off and the nature of the results allows for more discrimination between individual results.